Nope, can t do it, this is too much to count 0,050,000 to 41,050,000 /- 50,000.11 still great sorry, still too hard to count 1:1,000,000 42 41,500,000 to 41,500,000 /- 500,000.1 not too bad easy to count,000,000 4 35,000,000 to 45,000,000 /-.
First of all, we re going to use the Viable Plate Count method - the only method that can tell a live cell from a dead one for a rundown on counting methods, look here.
The prince looked at the mushy glutinous mass in his mother s cauldron with dismay.I need a hint :Assume you are starting with 1,000,000,000 cells in one mL of solution another hint :After first dilution you are left with 10,000,000 cells in one mL of solution dilution factor 0 another hint :Dilution factor is the same each time another.But saying Americans use 304,339,335,805,780 bags/yr is ridiculous, and it running man episode 106 makes you sound like a pompous know-it-all to boot.This method is called multiplying by the inverse of the dilution factor.Why do we dilute.When I add a small amount of the concentrated stuff to an empty container, top it off with saline/water/whatever, then remove some small amount, this constitutes a dilution.
I think I have the answer: 0,000,000.
In these examples, the numbers will always come out evenly if you choose dilutions of 1/2, 1/5, 1/10, or their multiples.
For example, we could take super-strong cofee, dilute it by 1/5, then dilute that by 1/10.But by definition, we re counting only a fraction of what was originally there.The prince gave it a try and promptly spit it back out again.I need a hint :Assume that you are starting with 20,000 cells in one mL of solution another hint :After first dilution you are left with 400 cells in one mL of solution 20,000/50 another hint :After second dilution you are left with 8 cells.If your original solution contained 50,000,000 bacteria: the,000 sample will contain about 5,000 CFUs.Let s look at this in graphical form: Simple, right.The first problem should be very easy at this point, but after that you can click on Hard Problem to get trickier dilution schemes.Why would you want to do this.I ve made your favorite, cooed his mother unconvincingly, split pea soup.